mouse granzyme b elispot kit Search Results


mouse elispot kit  (R&D Systems)
92
R&D Systems mouse elispot kit
Mouse Elispot Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+granzyme+b+elispot+kit/Mouse+Granzyme+B+ELISpot+Kit/pmc03624290-127-11-14
Average 92 stars, based on 1 article reviews
mouse elispot kit - by Bioz Stars, 2026-10
92/100 stars
  Buy from Supplier
elispot mouse granzyme b kit  (R&D Systems)
92
R&D Systems elispot mouse granzyme b kit
Figure 3. Change in tumor cell phagocytosis by BMDCs and IFN-g secretion or granzyme release by mixed lymphocytes after tumor cell loss of CD47. (a) Phagocytic uptake of CFSE labeled HPV(1) cells was analyzed by incubating BMDCs and shRNA non-silencing control (control) or stable shRNA-mediated CD47 knockdown (shCD47) HPV(1) MTEC cells. After CFSE treatment, HPV(1) MTEC cells were incubated with BMDCs for 18 hr and analyzed by fluorescence microscopy and FACS analysis. Percent of dendritic cells with phagocytic CFSE uptake is shown in upper right of graph. Graphs were obtained by FACS analysis for MHC-II and CFSE expression. Representative images of dendritic cells are shown with arrows, indicating the CFSE uptake in the BMDCs. Scale bar, 200 mm. (b) Mixed lymphocytes were harvested from the draining inguinal node of tumor-bearing mice and incubated with the indicated HPV(1) MTEC cells: shRNA nonsilencing control (control), irradiated control (30 Gy) or shRNA-mediated CD47 knockdown (shCD47). IFN-g secretion was assayed by commercial ELISA from the supernatant after 18 hr. (c) <t>ELISpot</t> Granzyme B assay of splenocytes harvested from null-vaccinated (na€ıve) or HPV 16 E6E7-vaccinated (activated) mice. Splenocytes were harvested and evaluated for reactivity toward nonsilencing control or shCD47 HPV(1) MTEC cells through secretion of granzyme B. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
Elispot Mouse Granzyme B Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+granzyme+b+elispot+kit/Mouse+Granzyme+B+ELISpot+Kit/pm23292955-87-55-60
Average 92 stars, based on 1 article reviews
elispot mouse granzyme b kit - by Bioz Stars, 2026-10
92/100 stars
  Buy from Supplier

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Figure 3. Change in tumor cell phagocytosis by BMDCs and IFN-g secretion or granzyme release by mixed lymphocytes after tumor cell loss of CD47. (a) Phagocytic uptake of CFSE labeled HPV(1) cells was analyzed by incubating BMDCs and shRNA non-silencing control (control) or stable shRNA-mediated CD47 knockdown (shCD47) HPV(1) MTEC cells. After CFSE treatment, HPV(1) MTEC cells were incubated with BMDCs for 18 hr and analyzed by fluorescence microscopy and FACS analysis. Percent of dendritic cells with phagocytic CFSE uptake is shown in upper right of graph. Graphs were obtained by FACS analysis for MHC-II and CFSE expression. Representative images of dendritic cells are shown with arrows, indicating the CFSE uptake in the BMDCs. Scale bar, 200 mm. (b) Mixed lymphocytes were harvested from the draining inguinal node of tumor-bearing mice and incubated with the indicated HPV(1) MTEC cells: shRNA nonsilencing control (control), irradiated control (30 Gy) or shRNA-mediated CD47 knockdown (shCD47). IFN-g secretion was assayed by commercial ELISA from the supernatant after 18 hr. (c) ELISpot Granzyme B assay of splenocytes harvested from null-vaccinated (na€ıve) or HPV 16 E6E7-vaccinated (activated) mice. Splenocytes were harvested and evaluated for reactivity toward nonsilencing control or shCD47 HPV(1) MTEC cells through secretion of granzyme B. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Journal: International journal of cancer

Article Title: Radiation-induced loss of cell surface CD47 enhances immune-mediated clearance of human papillomavirus-positive cancer.

doi: 10.1002/ijc.28015

Figure Lengend Snippet: Figure 3. Change in tumor cell phagocytosis by BMDCs and IFN-g secretion or granzyme release by mixed lymphocytes after tumor cell loss of CD47. (a) Phagocytic uptake of CFSE labeled HPV(1) cells was analyzed by incubating BMDCs and shRNA non-silencing control (control) or stable shRNA-mediated CD47 knockdown (shCD47) HPV(1) MTEC cells. After CFSE treatment, HPV(1) MTEC cells were incubated with BMDCs for 18 hr and analyzed by fluorescence microscopy and FACS analysis. Percent of dendritic cells with phagocytic CFSE uptake is shown in upper right of graph. Graphs were obtained by FACS analysis for MHC-II and CFSE expression. Representative images of dendritic cells are shown with arrows, indicating the CFSE uptake in the BMDCs. Scale bar, 200 mm. (b) Mixed lymphocytes were harvested from the draining inguinal node of tumor-bearing mice and incubated with the indicated HPV(1) MTEC cells: shRNA nonsilencing control (control), irradiated control (30 Gy) or shRNA-mediated CD47 knockdown (shCD47). IFN-g secretion was assayed by commercial ELISA from the supernatant after 18 hr. (c) ELISpot Granzyme B assay of splenocytes harvested from null-vaccinated (na€ıve) or HPV 16 E6E7-vaccinated (activated) mice. Splenocytes were harvested and evaluated for reactivity toward nonsilencing control or shCD47 HPV(1) MTEC cells through secretion of granzyme B. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Article Snippet: ELISpot: mouse granzyme B assay Na€ıve mice were vaccinated with either Ad5 E6/E7 or Ad5 null virus and boosted 7 days postvaccination with the appropriate vaccine as described previously.37 Splenocytes were harvested on day 14 and after incubation with the indicated cells for 4 hr, granzyme release was assessed from 5 3 105 splenocytes using ELISpot Mouse Granzyme B kit (R&D Systems, Minneapolis, MN) and methods were followed as per the manufacturer’s instructions.

Techniques: Labeling, shRNA, Control, Knockdown, Incubation, Microscopy, Expressing, Irradiation, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot